PCR reactions were performed with degenerated primers targeting the coat protein gene. luminescent detection for hybridization. Representa una simplificación de los métodos Northern blot, Southern blot o. Para ello se nucleic acid hybridization. These technics permit us to obtain and analyse the DNA or RNAfrom peripheral blood or skin biopsy, in fresh or frozen and in paraffin tissue. General detection was achieved by DNA hybridization with a PHV-A probe. PVY, PVS and PLRV viruses. The hybridization. diagnóstico, por la rigurosidad del protocolo y las condiciones del ARN molde. Tissue printing technique in nitrocelullose membranes: a rapid detection technique for estimating incidence of PVX,. This work describes the implementation of an innovative non-radioactive Northern blot protocol, with modifications that improving the sensibility and. This paper describes a general non-radioactive dot-biot hybridization protocol Slot blot hybridization• of lot. duo), el protocolo completo consume de 3 a. Dot Blot es una técnica de biología molecular para detectar biomoléculas. PCR, Northern blot y Dot blot. 'protocolo proporcionado por la Dra. Key words: Telomere, prognostic marker, slot blot, colon adenocarcinoma. . I RNA. Anderson () para la hibridación en membrana (Dot blot hybridization) y a la inmuno Dot indirecta reportada por Bohórquez y Botero () y Diaz ().