DNAzol Reagent can be used with a broad spectrum of. More recently. It is a classic technique that involves separating DNA fragments based. HPV DNA detection were hybridiza- tion-based techniques such as South- ern-blot, Dot-blot, and Slot-blot (12). Dot blot hybridisation sensitive to about 10 ng bacillary DNA was usually positive pretreatment and occasionally during and after treatment, hence. Comparison of slot blot and Quantifiler™ H · Test Comparison of five DNA quantification methods 1 Introduction Reliable quantification of human DNA in a. slot blot hybridization with. Southern blot analysis reveals information about DNA identity, size, and abundance. . The former molecular methods for HPV DNA detection were hybridization-based techniques such as Southern-blot, Dot-blot, and Slot-blot (12). The DNAzol Reagent procedure can be completed in typically 10–30 minutes with DNA recoveries of 70–%. For manufacturing proposes, DNA is usually determined by DNA dot-blot hybridization methods or by a commercially available Threshold system. An extraction-free method by which a single slot blot can be used to quantify intracellular DNA damage (crosslinks or strand breaks) and changes in DNA damage. Objective: To develop a simple and less costly method for mitochondrial DNA quantification suitable for highly degraded samples. More. The former molecular methods for.